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KMID : 0379119890170030114
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Korean Journal of Mycology
1989 Volume.17 No. 3 p.114 ~ p.118
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Transfer of Isolated Nuclei from Agrocybe aegerita Mycelia into Pleurotus florida Protoplasts
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신평균/Shin, Pyung Gyun
장권열/유영복/차동열/변명옥/김한경/유차현/Chang, Kwon Yawl/Yoo, Young Bok/Cha, Dong Yeul/Byun, Myung Ok/Kim, Han Kyoung/You, Chang Hyun
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Abstract
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The transfer of isolated nuclei from Agrocybe aegerita mycelia of wild type into Pleurotus florida protoplasts of auxotroph was induced with polyethylene glycol. The type 1 of nuclear transfer products was spontaneous segregants of both parental morphology of colony. Hyphae of A. aegerita type had clamp connections while that of P. florida type lacked. Type 2 was main products of nuclear transfer which formed true clamp connections. Type 3 was clampless products. They all produced primordia and developed basidiocarps similar to Agrocybe aegerita.
A comparison of nuclei transfer products was made using isozyme analyses of alcohol dehydrogenase, esterase, lactate dehydrogenase and peroxidase. Isozyme band patterns of some type 2 strains produced a new band. Band patterns from mycelial extracts of the other strains could be characterized by parental bands.
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